Your Trusted Partner in Protein Interactions and Synthetic Nanobodies

We used Hybrigenics Y2H service for one of our root-knot nematode effectors. This is our first venture with Hybrigenics and our experience was fantastic. They undertook our project as a research collaboration with a goal to deliver us reliable/high quality data. They not only tested different strategies for cloning our bait but also decided to run the Y2H cycle with two different libraries in order to provide us with a more reliable list of interactors. Dr. Petra Tafelmeyer is very knowledgeable and helping and stayed involved in the project even after their job was done. She not only worked out the data analysis part with me but also helped me in designing the follow up experiments. We are looking forward to use Hybrigenics services in the future as well

Their system to investigate protein-protein interactions is excellent and have much contributed for our analyses of function of an Arabidopsis gene that appears to be involved in stress responses.

I was very impressed by the Hybrigenics ULTImate Y2H yeast-two-hybrid library screen service. The company worked closely with me to choose the best library and bait orientation for my gene based on my previous research findings. They also synthesized the gene for small cost, saving me the time and effort it would have taken to clone, sequence, and mail it. Most importantly, the interacting protein candidates they identified were very insightful and I have a lot of confidence in their results. Their method is superior to the standard (and time consuming) Y2H screen method I have done on my own with homemade libraries. Further, the Hybrigenics analysis pipeline eliminated false positives using data from previous screens. Further, their customer service was excellent starting with their response to my initial inquiry and continuing through to their detailed explanations of the results and their rankings. I was also kept updated on the progress of each step of the screen. I see no reason to perform my own Y2H screens in the future when Hybrigenics has a compatible plant library for my genes of interest. They were highly recommended to me by a colleague, and I would highly recommend their service to anyone interested.

Our interactions with the Hybrigenics team have exceeded expectations. We have performed over a half dozen screens with them and also partnered on the creation of new libraries. These interactions have resulted in significant preliminary data that has laid the foundation for multiple new and exciting projects in our laboratory. They are extremely professional, remarkably responsive, and technically proficient.

Hybrigenics was very professional helping us to design the best customized Y2H strategy for each one of our proteins, and were also flexible to try different approaches during the development of the project to maximize the chances of success.

My experience with Hybrigenics and your Y2H services was overwhelmingly positive. I was impressed with the lengths that Hybrigenics pursued to make our Y2H screen with MYC a success. Staff were in constant communication and consultation with us the entire way, and ultimately provided a set of novel high-confidence MYC interactors that we still work on to this day. We had performed our own Y2H screens with MYC in the past, but none even remotely compared to the quality and reliability of the screen with Hybrigenics.

Working with Hybrigenics staff is more like having a collaborator than a service provider…staff is very knowledgeable and highly responsive..their expertise and novel methodology gives high hit reliability.

Hybrigenics yeast two-hybrid screening is unparalleled in terms of completeness and quality of service. Moreover, their services are a tremendous value, as we could not set it up in our lab for even half the cost. Their screens of two of our baits have helped land one grant and will be the backbone for another.

I used Hybrigenics Y2H services to identify new protein-protein interactions using our protein of interest as a bait against their human placenta library. The results of the screening were very satisfying. They contained a detailed analysis of each hit and an estimation of their level of confidence which was helpful. Most of the putative interactors were found more than once suggesting that the screening was performed thoroughly. Importantly, one of the hits really fitted with our previous observations and, once validated, helped to delineate the mechanism of action that we had been struggling to find out. Thus, the screening results were very valuable to us and ultimately led to a publication in Science Signaling. The people at Hybrigenics were really helpful and efficient and I was extremely pleased with their performance. Hybrigenics provides a high quality service and I would strongly recommend it to other scientists.

My group has been using the services provided by Hybrigenics to perform y2H screens from Arabidopsis thaliana libraries using viral proteins as baits at several occasions, and has been very satisfied. Not only Hybrigenics teams have a great expertise in bait design, in performing high-coverage screens, and most importantly in scoring the candidates obtained and pinpointing the technical false-positives - which are invaluable assets, but I also found very enjoyable to work with them. They are friendly, supportive, and highly competent, and I always appreciated the discussions we had together. I will for sure never ever perform a yeast two-hybrid screen myself again and I strongly recommend their high-quality services, which are really worth the price. Moreover, following a very successful yeast two-hybrid screen using one of our viral protein as a bait, our group also got the chance to make use of their newly developed Hybribody service, i.e. synthetic single chains antibodies. Hybrigenics selected several VHH intrabodies, which they further validated in mammalian cells using fluorescence microscopy. Our lab is now currently validating them in plant cells and the preliminary data we obtained appear very promising. In addition to their use as basic research tools to detect our proteins in living cells, the intrabodies obtained may as well turn useful to inhibit key steps in the viral multiplication process, thus opening new possibilities for designing antiviral strategies.